KMID : 0545120190290020297
|
|
Journal of Microbiology and Biotechnology 2019 Volume.29 No. 2 p.297 ~ p.303
|
|
Dexamethasone Facilitates NF-¥êB Signal Pathway in TNF-¥á Stimulated Rotator Cuff Tenocytes
|
|
Ji Jong-Hun
Kim Young-Yul Patel Kaushal Cho Nam-Joon Park Sang-Eun Ko Myung-Sup Park Suk-Jae Kim Jong-Ok
|
|
Abstract
|
|
|
Corticosteroids are commonly used for pain control in rotator cuff tear. Deregulated NF-¥êB activation is a hallmark of chronic inflammatory diseases and has been responsible for the pathogenesis of rotator cuff tear. The Dexamethasone(DEXA) is a synthetic corticosteroid. The purpose of this study was to examine the exact effect of dexamethasone on NF-¥êB signaling in rotator cuff tear. We measured NF-¥êB expression in four groups: control, TNF-¥á-treated, DEXA-treated, and combined treatment with TNF-¥á and DEXA. Tenocytes were isolated from patients with rotator cuff tears and pre-incubated with TNF-¥á (10 ng/ml), DEXA (1 ¥ìM), or both of them for 10 min, 1 h, and 2 h. Expression of p65, p50, and p52 in the nuclei and cytosol was analyzed by western blotting and immunofluorescence imaging using confocal microscopy. We also evaluated nucleus/cytosol (N/C) ratios of p65, p50, and p52. In our study, the combined treatment with DEXA and TNF-¥á showed increased N/C ratios of p65, p50, and p52 compared with those in the TNF-¥á group at all time points. Additionally, in the DEXA group, N/C ratios of p65, p50, and p52 gradually increased from 10 min to 2 h. In conclusion, DEXA promoted the nuclear localization of p65, p50, and p52, but was not effective in inhibiting the inflammatory response of TNF-¥á-stimulated rotator cuff tear.
|
|
KEYWORD
|
|
Rotator cuff, tenocytes, NF-kappa B, glucocorticoid, tumor necrosis factor-alpha
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|